UV spectrophotometric method has been developed for simultaneous estimation of tramadol hydrochloride (TRM) and chlorzoxazone (CHZ) in bulk drug and in pharmaceutical dosage form. This method utilizes 0.1 N NaOH as a solvent and λmax of tramadol hydrochloride and chlorzoxazone selected for analysis was found to be 271 nm and 245 nm respectively. Linearity was observed in the concentration range of 30-90 μg/ml (TRM) (r2 = 0.998) and 6-14 μg/ml (CHZ) (r2 = 0.999) of both drugs. The accuracy and precision were determined and found to comply with ICH guidelines. This method showed good reproducibility and recovery with % RSD in the desired range. The proposed methods can be successfully applied for the routine analysis of both the drugs in marketed formulation. This method was simple, rapid, accurate and sensitive.
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